Proteomic study of the effect of recombinant hFGF-10 adenovirus on HaCat cells.
- Author:
Xiao-Ping WU
1
;
Yao-Ying ZENG
Author Information
1. Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
Cell Line;
Electrophoresis, Gel, Two-Dimensional;
Fibroblast Growth Factor 10;
genetics;
metabolism;
Humans;
Keratinocytes;
cytology;
metabolism;
Proteomics;
methods;
RNA, Messenger;
metabolism;
Recombinant Proteins;
metabolism;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
Transfection;
Up-Regulation;
Voltage-Dependent Anion Channel 2;
biosynthesis;
metabolism
- From:
Acta Pharmaceutica Sinica
2010;45(1):55-59
- CountryChina
- Language:Chinese
-
Abstract:
This study is to investigate the effect of recombinant hFGF-10 adenovirus on the proteome of HaCat cells, and to speculate further the possible mechanism of the effect of hFGF-10 on HaCat cells via differentially expressed proteins identified. Two-dimensional gel electrophoresis (2-DE) combined with tandem time-of-flight mass spectrometry was applied to identify the differentially expressed protein spots on the 2-DE maps of the whole-cell proteins from Ad infected and rAd-hFGF-10 infected HaCat cells. The mRNA and protein levels of the differentially expressed proteins were confirmed with semi-quantitative RT-PCR and Western blotting. The results showed that the 2-DE maps with high resolution were obtained, and four selected differentially expressed proteins involved in cell apoptosis, cytoskeleton regulation and protein degradation were identified with MALDI-TOF/TOF. The mRNA and protein levels of one of the differentially expressed proteins, VDAC2, were up-regulated in HaCat cells infected with the recombinant hFGF-10 adenovirus. The differentially expressed protein, VDAC2, may be related to the bioactivities of hFGF-10.
