Preparation and identification of Jujuboside A artificial antigen.

Author: Degeniee ¹ ; Wei-Ku ZHANG ² ; Hao-Yi QIAO ¹ ; Hui-Hua QU ³ ; Yan ZHAO ¹ ; Qing-Guo WANG ¹ ; Jie-Kun XU ¹
Author Information

1. School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China.
2. Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China.
3. Center of Scientific Research, Beijing University of Chinese Medicine, Beijing 100029, China.
Publication Type:Journal Article
Keywords: Jujuboside A; MALDI-TOF-MS; artificial antigen; monoclonal antibody; sodium periodate oxidation method
From: China Journal of Chinese Materia Medica 2016;41(10):1880-1883
CountryChina
Language:Chinese
Abstract: Immunogenic antigen (jujuboside A-BSA) and coating antigen (jujuboside A-OVA) of jujuboside A were synthesized by sodium periodate oxidation method for the first time. Jujuboside A artificial antigen was confirmed by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF-MS). The titer and specificity of the antibody in serum of immunized mice were detected by enzyme-linked immunosorbent assay (ELISA). The corrected relation curve of inhibition rate showed that the antibody against Jujuboside A obtained from immunized mice could bind to jujuboside A and the titer was up to 1∶4 000. The jujuboside A artificial antigen was synthesized, which can be used further to preparation of monoclonal antibody and the pharmacokinetics study of jujuboside A in laboratory animals.