Isolation of thermophilic bacteria Thermus sp. YBJ-1 and cloning of amylase gene.
- Author:
Peng-Jun XIONG
1
;
Jian-Jun WEN
Author Information
1. The Key Laboratory of Marine Biotechnology, The Third Institute of Oceanography, SOA, Xiamen 361005, China.
- Publication Type:Journal Article
- MeSH:
Amylases;
genetics;
Bacterial Proteins;
genetics;
Cloning, Molecular;
Culture Media;
Open Reading Frames;
genetics;
Thermus;
enzymology;
genetics;
isolation & purification
- From:
Chinese Journal of Biotechnology
2004;20(3):434-436
- CountryChina
- Language:Chinese
-
Abstract:
Thermophilic bacteria strain YBJ-1 was isolated from hot spring samples collected from Yangbajing, Tibet. The 16sr DNA sequence of YBJ-1 (1511bp in length) shares 98% identity with that of Thermus scotoductus strain ITI-252T. The full-length ORF of amylase gene of YBJ-1 (amyT) was amplified by PCR technique and cloned into T-vector. The complete sequence of amyT is 1767bp in length, coding for 588 amino acids. The deduced amino acids share 99% similarity with alpha-cyclodextrinse of Bacillus sterothermophilus, 96% with maltogenic amylse of Thermus. sp IM6501, and 81% with neopullulanase of Bacillus sterothermophlus.
