Influence of betahistine on the expression of histamine H3 receptor in the medial vestibular nucleus following unilateral labyrinthectomy in guinea pigs
10.3760/cma.j.issn.1673-0860.2014.12.013
- VernacularTitle:倍他司汀对豚鼠单侧迷路破坏后前庭内侧核组胺H3受体表达的影响
- Author:
Jingcheng SHU
1
;
Shihua YIN
;
Yuan LIU
;
Xiuli RAO
;
Shunlian WEI
Author Information
1. 广西医科大学第一附属医院耳鼻咽喉头颈外科
- Keywords:
Vestibular nuclei;
Receptors,histamine H3;
Betahistine;
Guinea pigs
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2014;49(12):1027-1030
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of betahistine on the expression of histamine H3 receptor in the medial vestibular nucleus (MVN) following unilateral labyrinthectomy (UL).Methods Fifty-six healthy guinea pigs were randomly divided into three groups:the sham-operated group (group Ⅰ),the UL group [group Ⅱ,and UL + betahistine (BET) group (group Ⅲ)],BET was intraperitoneally injection at 2.17 mg · kg-1 · d-1 for 7 days.The expression of histamine H3 receptor was analyzed by immunohistochemistry at 1 day,3 days and 7 days after UL.Results H3 receptors were presented in the MVN and the expression of histamine H3 receptor were increased significantly in the ipsilateral MVN at 1 and 3 days after UL(P ＜0.05),the change turned into the normal value at 7 days(P ＞0.05).In the UL + BET group,the intensity of histamine H3 receptor was lower than that in the UL at 1 day and 3 days(4.25 ± 0.71,3.50 ± 0.92 vs 5.75 ± 0.71,5.50 ± 0.93,P ＜ 0.05).However,the changes turned into the normal values at 3 and 7 days (P ＞ 0.05).Conclusions The early stage of the vestibular compensation process may be associated with the change of H3 receptor expression in MVN.In the UL + BET group the histamine H3 receptor recovered quickly.
