Expression and significance of NLRP3 inflammasome and its downstream factors IL-1β/IL-18 in rat model of allergic rhinitis
10.3760/cma.j.issn.1673-0860.2015.02.011
- VernacularTitle:NLRP3炎性小体及下游因子IL-1β/IL-18在变应性鼻炎大鼠模型中的表达及意义
- Author:
Huijuan WAN
1
;
Hongxia SU
;
Yuying WU
;
Yulin ZHAO
;
Minghui ZHOU
Author Information
1. 450052,郑州大学第一附属医院鼻科 河南省高等学校临床医学重点学科开放实验室
- Keywords:
Rhinitis,allergic;
Receptors,cytoplasmic and nuclear;
Caspase 1;
Interleukin-1 beta;
Interleukin-18;
Disease models,animal
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2015;50(2):145-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression and explore the role of the innate immune NLRP3 inflammasome and its downstream factors interleukin-1 β (IL-1β)/ interleukin-18 (IL-18) in rat model of allergic rhinitis (AR).Methods Forty Sprague Dawley (SD) rats were randomly divided into control group (A group),AR model group 1 (B group),AR model group 2 (C group),AR model group 3 (D group).Every group contained 10 rats.After the rats in the model group were sensitized by ovalbumin (OVA) and alum,B,C and D groups were separately stimulated with 5％ OVA for 10 days,20 days and 30 days (once a day).The control group did not add OVA in the process of sensitization and excitation.All rats were executed after excitation.Eosinophil granulocyte (EOS) infiltration were observed in nasal mucosa by hematoxylin-eosin (HE) staining,the expression of NLRP3 and cysteinyl aspartate-specific protease-1 (Caspase-1) were observed in nasal mucosa by immunohistochemical staining.The concentrations of ovalbumin specific IgE (OVA-sIgE),IL-18 and IL-1 β in peripheral blood and the concentrations of IL-18 and I L-1 β in nasal fluid were tested by enzyme-linked immunosorbent assay (ELISA).The data were processed by SPSS 17.0 software.Results EOS cell counted,the behavioral score and the concentrations of OVA-sIgE in AR model group were obviously higher than those in control group (P ＜ 0.05),and the difference of which had statistical significance between the AR model groups (P ＜ 0.05).The expression of NLRP3 in AR model group (The expression of NLRP3 in group of B,C and D were 48.80 ± 10.75,71.80 ± 16.98 and 100.32 ± 13.91,respectively)were obviously higher than those in control group (17.47 ± 5.59),the difference of which had statistical significance (F =78.399,P ＜0.05).The expression of Caspase-1 in AR model group (The expression of Caspase-1 in group of B,C and D were 36.33 ± 4.71,50.87 ± 11.18 and 73.10 ± 14.77,respectively) were obviously higher than those in control group (11.48 ±2.70),the difference of which had statistical significance (F =71.727,P ＜0.05).The concentrations of IL-1β in AR model group [The concentrations of IL-1β in group of B,C and D were (56.46 ± 10.13),(82.37 ± 11.93),(112.01 ± 22.91) pg/ml,respectively] were obviously higher than those in control group [(38.26 ±4.66) pg/ml],the difference of which had statistical significance (F =51.981,P ＜0.05).The concentrations of IL-18 in AR model group [The concentrations of IL-18 in group of B,C and D were (177.92 ± 23.63),(194.33 ± 20.78),(234.06 ± 31.70) pg/ml,respectively] were obviously higher than those in control group [(89.71 ± 5.56) pg/ml],the difference of which had statistical significance (F =73.295,P ＜ 0.05).And the difference of which had statistical significance between the AR model groups (P ＜ 0.05).The expression of NLRP3 was significantly positively correlated with the behavioral score,the concentrations of OVA-sIgE and EOS cell counted in rat model of allergic rhinitis (r value were 0.833,0.873 and 0.868,respectively,all P ＜0.01).Conclusion NLRP3 inflammasome and its downstream factors IL-1β/IL-18 play a role in the pathogenesis of allergic rhinitis,which may be correlated with the degree of inflammation.
