Study on discriminating nephrotoxic components in Zexie.
- Author:
Xiaoping ZHAO
1
;
Lin LU
;
Yufeng ZHANG
;
Boli ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Alisma; chemistry; toxicity; Animals; Chromatography, High Pressure Liquid; Kidney; drug effects; LLC-PK1 Cells; Mass Spectrometry; Swine
- From: China Journal of Chinese Materia Medica 2011;36(6):758-761
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo sift and identify the nephrotoxic components in Zexie for controlling the quality of the herb.
METHODThe fractions of zexie were prepared by Pre-HPLC, then the nephrotoxicity of the fraction was sifted using LLC-PK1 labelled with fluorescein diacetate and MTT assay. Finally, the compounds in the most obvious nephrotoxic fraction were identified with LC-MS.
RESULTUsing MTT and FDA assay, similar results were obtained. Fraction C13 was found to be the most toxic with FDA assay, in which three compounds, alisol C, 16, 23-oxido-alisol B and alisol O, were detected and characterized by multi -stage mass spectrometric analysis.
CONCLUSIONAlisol C, 16, 23-oxido-alisol B and alisol O in Zexie may cause nephrotoxicity.
