Induction of dendritic cells with multidrug resistance from K562/MDR1 cells.
- Author:
Li-xia SHENG
1
;
Xiao-bao XIE
;
Gui-fang OU-YANG
;
Yi WANG
;
Hui-ling ZHU
;
He HUANG
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; genetics; Cell Differentiation; drug effects; Dendritic Cells; cytology; Drug Resistance, Multiple; Granulocyte-Macrophage Colony-Stimulating Factor; pharmacology; Humans; Interleukin-4; pharmacology; K562 Cells; cytology; Transfection; Tumor Necrosis Factor-alpha; pharmacology
- From: Journal of Zhejiang University. Medical sciences 2011;40(5):489-494
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo induce the differentiation of K562/MDR1 cells into dendritic cells (DC) with multidrug resistance property.
METHODSK562/MDR1 cells and K562 cells were cultured in the presence of GM-CSF and IL-4 to generate DC and matured by TNF-α. On d14 K562/MDR1-DC and K562-DC cells were harvested and the expressions of CD1a, CD83, CD80, CD86, HLA-ABC and HLA-DR were assessed by flow cytometry (FCM). The antigen presentation function of K562/MDR1-DC and K562-DC was determined by allogenic mixed lymphocyte reaction (Allo-MLR). The expression of P-glycoprotein and the intracellular accumulation of daunorubicin (DNR) were detected by FCM. The sensitivity of K562/MDR1-DC and K562-DC cell to vincristine, adriamycin was measured using MTT assay.
RESULTSBoth K562/MDR1 and K562 cells were differentiated into dendritic cells in the presence of cytokine cocktails, showing the morphologic and immunophenotypic characteristics of DC. K562/MDR1-DC more markedly enhanced proliferation of allogeneic lymphocytes in MLR than K562-DC. High level expression of P-glycoprotein and efflux of DNR were demonstrated in K562/MDR1-DC. K562/MDR1-DC showed multidrug resistance property, with higher IC(50) to VCR and ADM than that of K562-DCs.
CONCLUSIONK562/MDR1 cells can be differentiated into DC with the presence of cytokines, the induced K562/MDR1-DC cells express high level of P-glycoprotein and acquire the multidrug resistance property.
