Alteration of mi-RNA expression profile after interferon treatment in HCV-infected Huh7.5.1 cells.
- Author:
Yan LU
1
;
Xiao-kang XING
;
Guang-zhong SONG
;
Zhi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Gene Expression Profiling; Hepacivirus; genetics; Humans; Interferon-alpha; pharmacology; Interferons; pharmacology; MicroRNAs; drug effects; genetics; RNA, Viral; genetics; Transfection
- From: Journal of Zhejiang University. Medical sciences 2011;40(6):598-602
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen the mi-RNA expression profile after interferon treatment in cells infected with hepatitis C virus (HCV).
METHODSHuh-7.5.1 cells was infected with HCV by in vitro transcription and cultured with interferon. The mi-RNA microarray was used to measure the mi-RNA expression in the control group, HCV transcription group and interference group. Intra-group differences were analyzed by the 2 ((-delt delt CT)) method.
RESULTSWith mi-RNA expressed in normal Huh-7.5.1 cells as a benchmark, expressions of 13 kinds of mi-RNAs were up-regulated after HCV infection and then down-regulated following interferon treatment; 7 were down-regulated after HCV infection and then up-regulated following interferon treatment.
CONCLUSIONmi-RNA10a, mi-RNA21, mi-RNA149, mi-RNA152 and mi-RNA210 may be related to hepatitis C virus replication and transcription.
