Expression Profile of lncRNA NONHSAT040475 in Peripheral Blood Leukocytes of Healthy Persons.
- Author:
Fei-Yan WANG
1
;
Lan LUO
1
;
Sha-Sha ZHAO
1
;
Hua-Ping WEI
1
;
Li WANG
1
;
Zhen-Yang GU
1
;
Li-Xun GUAN
1
;
Nan YANG
1
;
Zhe GAO
1
;
Li-Li WANG
1
;
Chun-Ji GAO
2
Author Information
- Publication Type:Journal Article
- MeSH: Flow Cytometry; Humans; Killer Cells, Natural; Leukocyte Count; Leukocytes; RNA, Long Noncoding
- From: Journal of Experimental Hematology 2016;24(4):1196-1200
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression profile of lncRNA NONHSAT040475 in peripheral blood leukocytes of healthy persons.
METHODSThe peripheral blood mononuclear cells were collected from 10 healthy volunteers, the CD3(+) T cells,CD19(+) B cells,CD56(+) NK cells and granulocytes were purified and sorted by flow cytometry. Then, the expression of lncRNA NONHSAT040475 in peripheral blood leukocyte subsets was detected by real-time quantitative PCR.
RESULTSthe expression levels of lncRNA NONHSAT040475 were various in lymphocyte subsets, its expression in B lymphocytes was significantly higher, as compared with T lymphocytes, while the expressions of lncRNA NONHSAT040475 in NK cells and granulocytes were relative low（P<0.01）.
CONCLUSIONlncRNA NONHSAT040475 is widely expressed in human peripheral blood leukocytes, and mainly expressed in B lymphocytes, therefore, laying the foundation for further study of B lymphocyte-related diseases. This study has brought a new opportunity for diagnosing and illustrating the molecular mechanism of hematologic disorder or autoimmune disease.
