Expression, purification and identification of St.Louis encephalitis virus-like particles in eukaryotic cells
10.3760/cma.j.issn.1003-9279.2011.06.002
- VernacularTitle:圣路易斯脑炎病毒样颗粒的真核表达、纯化与鉴定
- Author:
Xue GUO
1
;
Fu-Bin XING
;
Qing-Jie XIAN
;
Lin WANG
;
Peng-Fei YANG
;
Li-Ping ZHANG
;
Rui-Jin PING
;
Kong-Xin HU
Author Information
1. 长春生物制品研究所
- Keywords:
Encephalitis virus,St. Louis;
Fluorescence technology;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(6):405-408
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express St.Louis encephalitis virus-like particles in mammalian cells,it will provide a prerequisite for further immunological diagnostic studies.Methods 293T-cell were transiently transfected with recombinant PreM-E plasmid.Expression and antigenicity of the purified protein were determined by transmission electron microscope (TEM),Western-Blot,immunofluorescence assay and ELISA.Results Recombinant subviral particles,about 50 nm in diameter,were observed by TEM in the supernatant of transfected cells.The results of Westeru-Blot,IFA and ELISA showed the recombinant proteins retained immunoreactivity similar to those of native virus proteins.Conclusion St.Louis encephalitis viruslike particles has good antigenicity and physical appearance,It will provide a prerequisite for further immune diagnostic reagent.
