Human enterovirus 71 ( EV71 ) resisted type I interferon induced antiviral effect
10.3760/cma.j.issn.1003-9279.2012.02.008
- VernacularTitle:肠道病毒71型抵抗Ⅰ型干扰素诱导的抗病毒作用
- Author:
Yu CUI
1
;
Juau SONG
;
Qin-Qin SONG
;
Peng SUN
;
Xing GAN
;
Gong-Qi LI
;
Ke-Xia WANG
;
Jun HAN
Author Information
1. 安徽理工大学,医学院
- Keywords:
Enterovirus;
Interferon type Ⅰ;
Herpesvirus 1,human
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(2):102-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate human enterovirus 71 (EV71) resistance to type Ⅰ interferon induced antiviral effect.Methods After type Ⅰ interferons (α,β ) were incubated with HeLa cells,recombinant type Ⅰ herpes simple virus ( HSV-1 ) with green fluorescent protein (GFP) was inoculated onto the HeLa cells.HSV-1 proliferation was observed by GFP expression and PCR.After EV71 was inoculated onto HeLa cells incubated with the same quantity of interferon,proliferation of EV71 were detected by RTPCR of 2A gene. Results Recombinant HSV-1 GFP expression and viral DNA replication obviously decreased in HeLa cells incubated with type Ⅰ interferon (α,β).However,EV71 effectively proliferated in the interferon irritated HeLa cell by RT-PCR.Conclusion HeLa cell irritated by type Ⅰ interferon (α,β) produced antiviral substance that inhibits HSV-1 proliferation.EV71 resisted the antiviral substance induced by type Ⅰ interferon and could significantly replicate in the HeLa cells.
