Effect of magnetic nanoparticle of Fe3O4 combined with daunorubicin on lymphoma cell line Raji.
- Author:
Ping YANG
1
;
Jing WANG
;
Hong-Mei JING
;
Xiao-Yan KE
Author Information
1. Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Daunorubicin;
pharmacology;
Drug Resistance, Multiple;
Drug Resistance, Neoplasm;
Ferric Compounds;
pharmacology;
Gene Expression Regulation, Neoplastic;
Humans;
Magnetics;
Nanoparticles;
Transcription Factor RelA;
metabolism;
Tumor Suppressor Protein p53;
metabolism
- From:
Journal of Experimental Hematology
2011;19(2):385-389
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to investigate the effect of magnetic nanoparticle of Fe3O4 combined with daunorubicin on lymphoma cell line Raji. The Raji cells were cultured with Fe3O4-magnetic nanoparticle and daunorubicin polymerized by means of mechanical absorption, the cell viability was detected by MTT and trypan-blue exclusion; the apoptosis was detected by flow cytometry, the expressions of P53 and P65 are measured by Western blot. The results showed that raji cell proliferation rate was dramatically inhibited by daunorubicin and MNP-Fe3O4-DNR in concentration-and time-dependent manners, there was significant difference in OD (p < 0.05) between these 2 groups. The apoptotic rate was correlated with time and there was significantly different between these 2 groups (p = 0.05).Western blot showed that the grey value in daunorubicin and MNP-Fe3O4-DNR were 2.52 ± 0.32, 2.08 ± 0.39 in P65 and 0.685 ± 0.144, 0.870 ± 0.091 in P53 respectively (p < 0.05). It is concluded that the combination treatment of MNP-Fe3O4 and daunorubicin efficiently inhibits growth and induces apoptosis in Raji cell line, while the activation of P53 and down-regulation of NF-κB may play important roles.
