Effects of chronic trimetazidine treatment on atrial energy metabolism in a canine model of chronic atrial fibrillation
10.3321/j.issn:0253-3758.2008.06.019
- VernacularTitle:心房颤动犬能量代谢变化及曲美他嗪干预研究
- Author:
Wei HAN
1
;
Shu-Sen YANG
;
Na WEI
;
Hong HUO
;
Wei-Min LI
;
Hong-Yan ZHOU
;
Guo ZHOU
;
Yong CAO
;
Guo DONG
;
Song-Bin FU
Author Information
1. 哈尔滨医科大学第一临床医学院
- Keywords:
Trimetazidine;
Atrial fibrillation;
Energy metabolism;
Structural remodeling
- From:
Chinese Journal of Cardiology
2008;36(6):556-559
- CountryChina
- Language:Chinese
-
Abstract:
Objective Aim of the present study was to investigate the effect of ehronic trimetazidine treatment on atrial energy metabolism and endothelial function in a canine model of chronic atrial fibrillation (AF).Methods Eiighteen canines were randomly divided into sham.operated group(n=6),atrial pacing group(n=6),and trimetazidine group(n=6).In atrial pacing group and trimetazidine group,dogs were atrial paced at 400 beats per minutes for 6 weeks.Trimetazidine at 5mg·kg-1·d-1 was given one day before rapid atrial pacing for 6 weeks.Creatine phosphate (CrP),adenosine triphosphate(ATP).adenosine diphosphate (ADP) and adenosine monophosphate (AMP) in atrial tissue were analyzed by high-performance liquid chromatography.Total adenosine(TAN)Was calculated.The expression of endothetial nitric oxide synthase(eNOS)in atrial tissue was determined by Western blot and immunohistochemical staining.In addition,plasma levels of von WiUebrand factor(vWF) was quantified with enzyme-linked immunoadsorbent assay and NO2-/NO3-(NOx) was determined by nitrate reductase method.Results Atrial CrP(P<0.01) and CrP/ATP were significantly decreased in paced atrium compared to atrium from sham-operated group(P<0.05) while ATP,ADP,AMP and TAN remained unchanged (all P>0.05).Plasma vWF Was significantly increased and plasma NOx significantly decreased in paced animals compared to sham-operated animals.Atrial expression of eNOS Was also significantly reduced in paced animals(P
