Comparison between MLO-Y4 osteocyte and osteoblast to support osteoclast formation in vitro
10.3760/cma.j.issn.1002-0098.2010.07.010
- VernacularTitle:骨细胞与成骨细胞诱导破骨细胞分化的对比研究
- Author:
Liang CUI
1
;
Xiao-Tong LI
;
Yan-Qi YANG
;
Min-Kui FU
;
Ding ZHANG
Author Information
1. 中国医学科学院中国协和医科大学北京协和医院
- Keywords:
Osteocytes;
Osteoblast;
Osteoclast;
Vitamin D3
- From:
Chinese Journal of Stomatology
2010;45(7):411-415
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare between MLO-Y4 osteocyte and osteoblast to support osteoclast formation in co-culture system.Methods MLO-Y4 cells and murine osteoblast cells were co-cultured with bone marrow cells with or without vitamin D3 presence.Bone marrow cells were as control group.Tartrat resistant acid phosphatase(TRAP) + giant cells with three or more nuclei were counted and compared under a microscope at day 9.Results In the absence of vitamin D3,(1963.3±93.1)/plate osteoclasts were observed when MLO-Y4 cells co-cultured with bone marrow cells in 24-well plate.While only (12.7±5.5)/plate osteoclasts were found in the osteeblast group,and (6.0±1.O)/plate in control group.The statistical difference occurs for any two groups(P <0.05).Vitamin D3 could significantly increase osteoclast formation in the three groups.Conclusions Osteocytes could induce osteoclastogenesis without the presence of vitamin D3 and vitamin D3 could enhance the induction effects of MLO-Y4 and ostenblast cells.
