Effects of sunitinib malate on growth of human bladder transitional cell line T24 in vitro.
- Author:
Jin WEN
1
;
Han-zhong LI
1
;
Zhi-gang JI
1
;
Jing JIN
2
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Carcinoma, Transitional Cell; metabolism; pathology; Cell Line, Tumor; Fas Ligand Protein; metabolism; Humans; In Vitro Techniques; Indoles; pharmacology; Poly(ADP-ribose) Polymerases; metabolism; Pyrroles; pharmacology; Urinary Bladder Neoplasms; metabolism; pathology; Wound Healing; drug effects; fas Receptor; metabolism
- From: Chinese Medical Sciences Journal 2015;30(1):51-55
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the growth-inhibitory effect of sunitinib malate on human bladder transitional cell carcinoma (TCC) in vitro.
METHODSHuman bladder TCC cell line T24 was cultured and exposed to graded concentrations of sunitinib malate for 72 hours in vitro to determine the sensitivities to drug. Cell viability was measured by MTT assay. Cell apoptotic morphology was observed by fluorescence microscope following DAPI staining. Band expressions of Fas, Fas ligand, poly (ADP-ribose) polymerase (PARP) and β-actin were analyzed by Western blot. Wound healing process of T24 cells exposed to sunitinib malate was assayed.
RESULTSSunitinib malate exerted a concentration-dependent and time-dependent inhibitory effect on the T24 cell lines. Fluorescence microscopy showed that small vacuoles appeared in the nuclei of T24 cells and the vacuoles were bigger with higher drug concentrations. The expressions of Fas ligand and PARP in T24 cells treated with sunitinib malate exhibited a concentration-dependent increase. Moreover sunitinib malate suppressed the wound healing process in a concentration-dependent manner.
CONCLUSIONSunitinib malate exerted marked inhibitory activity against bladder cancer cell line T24.
