Cloning of human monoclonal Fab fragments against HIV-1 gp120 peptide binding chemokine receptor from phage Fab antibody library.
- Author:
Honghui SHEN
1
;
Panyong MAO
;
Shiwen HONG
;
Jun HOU
;
Jianyang YANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Antibodies, Monoclonal; genetics; Antibodies, Viral; genetics; Bacteriophages; genetics; Cloning, Molecular; HIV Envelope Protein gp120; Humans; Immunoglobulin Fab Fragments; genetics; In Vitro Techniques; Molecular Sequence Data; Peptide Library; Receptors, Chemokine; metabolism; Receptors, HIV; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(4):357-360
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen human monoclonal Fab fragments against HIV-1 gp120 peptide binding chemokine receptor.
METHODSA synthesized polypeptide containing 23 amino acid residues of the gp120 antigen epitope binding chemokine receptor was coated as the solid-phase antigen. After biopanning from the HIV-1 phage Fab antibody library, the acquired positive clones were tested and sequenced.
RESULTSOne clone of human phage Fab monoclonal antibody against HIV-1 gp120 polypeptide was acquired. It has high affinity, specificity and inhibition rate and it belongs to IgG I subclass and kappa type. Its Vh H and V kappa were derived from Vh III and V kappa III.
CONCLUSIONSThe human phage Fab fragment against HIV-1 gp120 antigen site binding chemokine receptor was acquired.
