Separation, purification and antioxidant activity of polysaccharide from Coprinus comatus.

Author: Nü XU ¹ ; Tianzhen ZHANG ¹ ; Xufeng CHEN ¹ ; Hao ZHANG ¹ ; Rufu WANG ¹
Author Information

1. Food Science and Engineering College, Shanxi Agricultural University, Taigu 030801, Shanxi, China.
Publication Type:Journal Article
Keywords: Coprinus comatus polysaccharides; antioxidation; physico-chemical property; purification
From: Chinese Journal of Biotechnology 2017;33(5):808-816
CountryChina
Language:Chinese
Abstract: We compared the ways of deproteinization for crude polysaccharides of Coprinus comatus, and finally selected Sevage method as the optimal method. Two main fractions of Ccp-I-A and Ccp-I-B were obtained after DEAE-52 cellulose and Sephadex G-200 chromatography, both were white-floc, soluble in water, insoluble in absolute ethyl alcohol, acetone and other organic solvents. Additionally, Fehling reagent, CTAB, Sulphuric acid-carbazole, I-KI and FeCl₃ reaction were all negative. GC analysis showed Ccp-I-A was composed of mannitose, glucose and galactose in molar ratios of 2.03:9.52:1, whereas Ccp-I-B was composed of fucose and galactose with molar ratios of 1:5.21. Antioxidant activity test showed that Ccp-I-A and Ccp-I-B had good scavenging abilities on DPPH and ·OH. Compared to Ccp-I-B,the scavenging activity of Ccp-I-A was much stronger, and the scavenging rate could reach 72.1% and 55.3% respectively when the concentration was 300 μg/mL.