Detection of epidermal growth factor receptor gene mutation in non-small cell lung cancer by allele-specific oligonucleotide-PCR and bi-loop probe specific primer quantitative PCR.
- Author:
Lei DENG
1
;
Yuan TANG
;
Jing LIN
;
Xiao-jun LU
;
Jian-xin XUE
;
Li-shuai WANG
;
Lin ZHOU
;
Yan ZOU
;
Bin-wu YING
;
Gan-di LI
;
You LU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; Carcinoma, Non-Small-Cell Lung; genetics; DNA Mutational Analysis; Female; Genes, erbB-1; Humans; Lung Neoplasms; genetics; Male; Middle Aged; Mutation; Polymerase Chain Reaction; methods; Receptor, Epidermal Growth Factor; genetics; Sensitivity and Specificity; Sex Factors; Smoking
- From: Chinese Journal of Pathology 2012;41(1):20-22
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the detection sensitivity of epidermal growth factor receptor (EGFR) mutations between allele specific oligonucleotide PCR (ASO-PCR) and bi-loop probe and specific primer quantitative PCR (BPSP-qPCR).
METHODSA total of 96 non-small cell lung cancer specimens were selected from West China Hospital from September 2009 to December 2010. ASO-PCR was developed to detect the presence of classical EGFR mutations. A total 39 available specimens were also tested by BPSP-qPCR.
RESULTSEGFR mutation detection rate was 30.2% (26/96) by ASO-PCR. The mutation rate was higher in female than in male patients [45.5% (20/44) vs. 17.3% (9/52), P = 0.003], non-smokers than smokers [44.1% (26/59) vs. 8.1% (3/37), P < 0.001] and adenocarcinomas than other subtypes of lung cancer [37.0% (27/73) vs. 8.7% (2/23), P = 0.01]. Among mutation negative cases by ASO-PCR, BPSP-qPCR increased the rate of detection of 19-del and L858R mutation by 10.3% (4/39) in adenocarcinomas and non-smoking subset. Overall, the mutation detection rate of BPSP-qPCR was higher than that of ASO-PCR [66.7% (26/39) vs. 41.0% (16/39), P = 0.02].
CONCLUSIONBPSP-qPCR has a better detection sensitivity than that of ASO-PCR.
