Evaluation of sperm mitochondrial membrane potential in varicocele patients using JC-1 fluorescent staining.
- Author:
Yi HU
1
;
Xin-yi XIA
;
Lian-jun PAN
;
Nian-qing LÜ
;
Yong-ming WU
;
Xin ZHOU
;
Xue-jun SHANG
;
Ying-xia CUI
;
Yu-feng HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Flow Cytometry; Humans; Male; Membrane Potential, Mitochondrial; Sperm Motility; Spermatozoa; physiology; Varicocele; metabolism; physiopathology; Young Adult
- From: National Journal of Andrology 2009;15(9):792-795
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect sperm mitochondrial membrane potential (MMP) of varicocele patients and investigate its clinical significance.
METHODSSixty-seven varicocele patients were divided into a VC1 (grade 1, n = 26), a VC2 (grade 2, n = 21) and a VC3 group (grade 3, n = 20). And 29 normal fertile volunteers were included in a control group ( m = 29). Conventional semen analyses were performed by computer-assisted semen analysis (CASA). Semen samples were washed, followed by JC-1 staining to evaluate the sperm MMP (JC-1+ %) by flow cytometry.
RESULTSThe sperm MMPs of the VC1, VC2 and VC3 groups were siginificantly lower ([56.29 +/- 16.32]%, P < 0.05; [45.04 +/- 13.21]%, P < 0.01; [31.63 +/- 12.91]%, P < 0.01) than that of the control ([76.21 +/- 13. 96]%). There was a significant positive correlation between the percentage of JC-1+ and that of grade (a + b) sperm (r =0.693, P=0.000).
CONCLUSIONThe decreased MMP in the sperm of varicocele men might be one of the important causes of male infertility.
