Tetramethoxystilbene, a selective CYP1B1 inhibitor, suppresses adipogenesis of C3H10T1/2 pluripotent stem cells.
- Author:
Cui-Fang FAN
1
;
An-Na ZHU
;
Ting-Ting HUANG
;
Lu LI
;
Su-Qing WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adipogenesis; drug effects; Animals; Cell Differentiation; drug effects; Cells, Cultured; Cytochrome P-450 CYP1B1; Cytochrome P-450 Enzyme Inhibitors; pharmacology; Mesenchymal Stromal Cells; cytology; drug effects; Mice, Inbred C3H; PPAR gamma; metabolism; Pluripotent Stem Cells; cytology; drug effects; RNA, Messenger; Stilbenes; pharmacology
- From: Journal of Southern Medical University 2015;35(1):72-76
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibitory effects of tetramethoxystilbene, a selective CYP1B1 inhibitor, on adipogenic differentiation of C3H10T1/2 multi-potent mesenchymal cells.
METHODSIn vitro cultured C3H10T1/2 cells at full confluence were induced by adipogenic agents (10 µg/ml insulin, 2 µmol/L dexamethasone and 0.5 mmol/L 3-isobutyl-1-methylxanthine) and exposed simultaneously to TMS at the final concentrations of 1.0, 2.0 or 4.0 µg/ml. Oil Red-O staining was used to observe the cell differentiation. The expression of peroxisome proliferator-activated receptor gamma (PPARγ) and its target genes cluster of differentiation 36 (CD36) and fatty acid binding protein 4 (FABP4) were quantified by real-time RT-PCR and Western blotting.
RESULTSOil Red-O staining and TG contents revealed that TMS suppressed induced differentiation of C3H10T1/2 cells. TMS exposure of the cells dose-dependently decreased both mRNA and protein expressions of PPARγ, a key nuclear transcription factor during adipogenesis, and also lowered the mRNA expressions of PPARγ target genes CD36 and FABP4.
CONCLUSIONTMS can suppress adipogenic differentiation of C3H10T1/2 cells by inhibiting PPARγ
