The Expression of Endothelin Receptor B in Melanoma Cells A375 and Sk-mel-1 and the Proliferative Effects of Endothelin 3 on A375 Cells
- Author:
Nengxing LIN
1
;
Changzheng HUANG
;
Jin TIAN
;
Juan TAO
;
Jin ZHANG
;
Lingyun YANG
;
Yan LI
;
Yeqiang LIU
;
Siyuan CHEN
;
Guanxin SHEN
;
Jiawen LI
;
Chunsen WANG
;
Yating TU
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
melanoma;
endothelin receptor B;
endothelin 3;
A375;
SK-mel-1
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(5):611-613
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the expression of endothelin receptor B (ETR-B) in human ma-lignant melanoma (MM) cells A375 and SK-mel-1 and the proliferative effects of endothelin 3 (ET3) on A375 cells, RT-PCR was applied to detect the expression of ETR-B gene in human MM cells A375 and SK-mel-1. MTT method was used to evaluate the growth enhancing effects of ET3 on A375 cell line in vitro. The results showed that ETR-B gene was expressed in both MM A375 and SK-mel-1 cells. ET3 had stronger ability to enhance the proliferation of A375 cells in vitro in a con- centration-dependent manner. It was suggested that ET3/ETR-B might play an important proliferative role in MM.
