Effect of 1,25-(OH)2D3 on expression of TIM-4 in the lungs of asthmatic mice.
- Author:
Bin LUAN
1
;
Ya-Zhe WANG
;
Yan-Li ZHANG
;
Hui-Ru GU
;
Yan-Ling LI
;
Jie ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Airway Remodeling; Animals; Asthma; metabolism; Calcitriol; pharmacology; Female; Gene Expression Regulation; drug effects; Lung; metabolism; Membrane Proteins; analysis; genetics; physiology; Mice; Mice, Inbred BALB C; RNA, Messenger; analysis
- From: Chinese Journal of Contemporary Pediatrics 2013;15(1):67-70
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a mouse model of asthmatic airway remodeling and investigate the effects of 1,25-(OH)2D3 on airway structure and T cell immunoglobulin mucin protein-4 (TIM-4) expression in asthmatic mice.
METHODSThirty female mice (BALB/c strain) were randomly divided into control, asthma and 1,25-(OH)2D3 intervention groups. An asthmatic mouse model was induced using ovalbumin. Lung tissue of the mice was collected, mRNA expression of TIM-4 was evaluated by RT-PCR and airway remodeling and protein expression of TIM-4 were observed by hematoxylineosin staining and immunohistochemistry.
RESULTSTypical airway remodeling was found in the asthma group, and TIM-4 expression in this group was significantly higher than in the control group (105±9 vs 42±5; P<0.05). Compared with the asthma group, the 1,25-(OH)2D3 intervention group showed improvement in airway remodeling and a decrease in TIM-4 expression (78±6) (P<0.05).
CONCLUSIONSTIM-4 may be involved in the airway remodeling of mice. As a new type of immunoregulator, 1,25-(OH)2D3 can downregulate expression of TIM-4 in the lungs and improve airway remodeling in asthmatic mice.
