Anti-angiogenesis effect on glioma of attenuated Salmonella typhimurium vaccine strain with flk-1 gene.
- Author:
Keke FENG
1
;
Hongyang ZHAO
;
Jian CHEN
;
Dongxiao YAO
;
Xiaobing JIANG
;
Wei ZHOU
Author Information
1. Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and technology, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Angiogenesis Inhibitors;
pharmacology;
Animals;
Brain Neoplasms;
blood supply;
therapy;
Female;
Glioma;
blood supply;
therapy;
Mice;
Mice, Inbred C57BL;
Neovascularization, Pathologic;
Salmonella Vaccines;
immunology;
pharmacology;
Salmonella typhimurium;
immunology;
Transformation, Genetic;
Vaccines, Attenuated;
genetics;
immunology;
pharmacology;
Vascular Endothelial Growth Factor Receptor-2;
genetics;
immunology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(4):389-391
- CountryChina
- Language:English
-
Abstract:
To investigate the anti-vasculature effects and the anti-glioma effects of attenuated Salmonella typhimurium vaccine strain expressing VEGFR2 (flk-1) gene, plasmid pcDNA3.1-flk1 was constructed and electro-transfected into live attenuated Salmonella typhimurium strain SL7207. Mouse models of intracranial G1261 glioblastoma were treated with an orally administered attenuated Salmonella typhimurium expressing flk-1 gene. The survival period was recorded and vessel density was observed by immunofluorescence. CTLs activity was measured by MTT assay. Our results showed that attenuated Salmonella typhimurium vaccine strain expressing flk-1 gene could significantly inhibit glioblastoma growth, reduce vessel density, prolong the survival period and improve the survival rate in these mice. The flk-1 specific CTLs activity was increased obviously after the vaccination. Our study showed that attenuated Salmonella typhimurium vaccine strain expressing flk-1 gene could break peripheral immune tolerance a in glioma gainst this self-antigen and kill endothelial cells by the orally administered vaccine and can be used for both prophylactic and therapeutic purposes.
