Preparation, characterization and Calu-3 cellular uptake of three kinds of poly(b-benzyl-L-amino)block-poly(ethylene glycol) nanoparticles.
- Author:
Yin ZHOU
1
;
Li-Na LU
;
Xue XIN
;
Dong-Feng HUO
;
Hong-Bing WU
;
Ming-Feng QIU
Author Information
1. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
- Publication Type:Journal Article
- MeSH:
Adenocarcinoma;
metabolism;
pathology;
Administration, Intranasal;
Anti-Inflammatory Agents, Non-Steroidal;
administration & dosage;
metabolism;
Aspartic Acid;
chemistry;
toxicity;
Cell Line, Tumor;
Cell Survival;
drug effects;
Curcumin;
administration & dosage;
metabolism;
Drug Carriers;
Ethylene Glycol;
chemistry;
toxicity;
Humans;
Lung Neoplasms;
metabolism;
pathology;
Lysine;
chemistry;
toxicity;
Nanoparticles;
Particle Size;
Polyethylene Glycols;
chemistry;
toxicity;
Polyglutamic Acid;
analogs & derivatives;
chemistry;
toxicity
- From:
Acta Pharmaceutica Sinica
2013;48(4):560-565
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper is to compare the cytotoxicity and cellular uptake efficiency of three kinds of poly(b-benzyl-L-amino) block-poly(ethylene glycol) nanoparticles (PXA-PEG-NPs) using Calu-3 cells, and select one as a nasal drug delivery vector for curcumin (Cur). Poly(gamma-benzyl-L-glutamate) block-poly(ethylene glycol) nanoparticles (PBLG-PEG-NPs), poly(gamma-benzyl-L-lysine) block-poly(ethyleneglycol) nanoparticles (PZLL-PEG-NPs) and poly(gamma-benzyl-L-aspartate) block-poly(ethylene glycol) nanoparticles (PBLA-PEG-NPs) were prepared by emulsion-solvent evaporation method. MTT assays were used to evaluate the cytotoxicity of PXA-PEG-NPs against Calu-3 cells. The cellular uptake of nanoparticles was visualized by an inverted fluorescence microscope and quantified by a flow cytometer. The results indicated that even at high concentration of 2 mg x mL(-1) the three nanoparticles had no cytotoxicity on Calu-3 cells. Compared to the curcumin solution, the three curcumin-loaded PXA-PEG-NPs showed significantly higher cellular uptake efficiency on Calu-3 cells (at equal concentration of curcumin with 5 microg x mL(-1) Cur solution), PBLG-PEG-NPs group was the highest. The cellular uptake increased with incubation time, and has positive correlation with nanoparticle concentration. In brief, PXA-PEG-NPs are conducive to delivery Cur into cells, and PBLG-PEG-NPs might be provided as a good nasal drug delivery carrier.
