Preparation and initial application of a monoclonal antibody specific for a newly discovered conserved linear epitope of rabies virus nucleoprotein.
- Author:
Xin Jun LV
1
;
Xue Jun MA
;
Li Hua WANG
;
Hao LI
;
Xin Xin SHEN
;
Peng Cheng YU
;
Qing TANG
;
Guo Dong LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; isolation & purification; Cell Line; Cricetinae; Dogs; Epitopes; Fluorescein-5-isothiocyanate; Fluorescent Dyes; Hybridomas; Mice; Mice, Inbred BALB C; Nucleoproteins; immunology; Rabies virus; immunology; Viral Proteins; immunology
- From: Biomedical and Environmental Sciences 2012;25(1):98-103
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo prepare monoclonal antibodies against a newly discovered and conserved linear epitope of Rabies virus nucleoprotein and to use them in a rabies diagnostic test.
METHODSSynthetic peptide containing the epitope was used as immunogen to prepare hybridoma cell lines by classical hybridoma technology. Anti-peptide monoclonal antibodies produced in ascites of inoculated Balb/c mice were labeled with fluorescein isothiocyanate (FITC) after purification and used in fluorescent antibody test (FAT).
RESULTSTwo positive hybridoma cell lines, RVNP-mAb1-CL and RVNP-mAb2-CL, were obtained. RVNP- mAb1-CL produced a higher concentration of monoclonal antibody RVNP-mAb1 in Balb/c ascites. FITC-labeled RVNP-mAb1 showed correct results on certain Rabies virus-positive canine brain tissue samples and cells of a small subclone of baby hamster kidney 21 cell line (BSR).
CONCLUSIONFITC-labeled RVNP-mAb1 has potential application for laboratory diagnosis of rabies.
