Regulation of E-cadherin promoter activity by LMO2 impact on the progression and metastasis of prostate cancer.
- Author:
Xi PENG
1
;
Zheng YE
1
;
Yang GAO
1
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; genetics; metabolism; Cadherins; genetics; Cell Line, Tumor; Genetic Vectors; Humans; LIM Domain Proteins; genetics; metabolism; Male; Neoplasm Metastasis; Point Mutation; Promoter Regions, Genetic; Prostatic Neoplasms; genetics; metabolism; pathology; Proto-Oncogene Proteins; genetics; metabolism
- From: Chinese Journal of Applied Physiology 2013;29(5):385-389
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the abnormal expression of the proto-oncogene LMO2 affect the progression and metastasis mechanism of prostate cancer.
METHODSA series of reporter gene expression vectors carrying different lengths and point mutations of E-cadherin promoter were constructed. These plasmids were separately co-transfected with LMO2 into Lncap cells and the luciferase activity was detected after 24 h.
RESULTSThe overexpression of LMO2 could significantly inhibit the activity of luciferase reporter gene of E-cadherin promoter about 50%. Truncated and point mutation study showed that this was mainly through E-box sites in the promoter region -204/-198.
CONCLUSIONThe proto-oncogene LMO2 can affect the progression and metastasis mechanism of prostate cancer by transcriptional inhibition of E-cadherin.
