Construction of Antisense MT1-MMP Vector and Its Inhibitory Effects on Invasion of Human Ovarian Cancer Cells
- Author:
Mingfu WU
1
;
Yanyan SHI
;
Lin XI
;
Qiong LI
;
Guo-Nin LIAO
;
Zhi-Qiang HAN
;
Yun-Ping LU
;
Ding MA
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
membrane type 1 matrix metalloproteinase (MT1 MMP);
ovarian tumor;
invasiveness
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(6):715-717
- CountryChina
- Language:Chinese
-
Abstract:
Membrane-type 1 matrix metalloproteinase (MT1 MMP/MMP 14) plays crucial roles in tumor cell growth, invasion, and angiogenesis. To clarify whether the endogenously expressed MT1-MMP in metastatic human ovarian carcinoma cell lines SKOV3 plays a critical role in tumor cell invasiveness, antisense MT1-MMP cloned in eukaryotic expression vector pMMP14as was transferred into SKOV3 cells. 48h after transfection, decreased expression of endogenous MT1-MMP protein was detected in pMMP14as transfected SKOV3 cells and the activation of pro MMP2was inhibited markedly. The mean percentage of invasive cells was (62. 50 ±5. 30) % in pMMP14as-transfected cells, which was obviously less than that (97.20±6.90) % in the control.Thus, antisense MT1 MMP effectively inhibited the endogenous MT1 MMP expression and the invasiveness in SKOV3 cells, suggesting that MT1-MMP may be a therapeutic target molecule for human invasive ovarian cancers.
