Effect of fast capacitance compensation method on improving the action potential firing accuracy of nerve cell.
- Author:
Yu ZHENG
;
Jing LI
;
Di CAI
;
Lei TIAN
;
Chaochao HUANG
;
Jinhai WANG
- Publication Type:Journal Article
- MeSH:
Action Potentials;
Electric Capacitance;
Ion Channels;
physiology;
Membrane Potentials;
Neurons;
cytology;
Patch-Clamp Techniques
- From:
Journal of Biomedical Engineering
2014;31(6):1191-1217
- CountryChina
- Language:Chinese
-
Abstract:
Patch-clamp is used to study all sorts of ionic channels and their regulations with measuring pA current of cell ionic channel, but the fast capacitance (C-fast) compensation and slow capacitance (C-slow) compensation transient currents are caused by measuring objects and measuring instruments themselves which will change the properties of action potentials. The present paper firstly discusses the C-Fast transient currents affecting membrane capacitance and membrane potential, and then draws a conclusion that the changes of membrane potential affect the properties of action potential through analyzing the changes of membrane potential in H-H model. Based on this conclusion, we discuss the influence mechanisms mainly through the analysis of traditional C-fast compensation errors, and focus discussion on the shape of electrode capacitance affecting C-fast. This method can not only improve the compensation speed greatly, but also improve the compensation precision from the electrode shape as much as possible.
