Association of FGFR2 gene polymorphism with estrogen receptor positive breast cancer detected by fluorescent quantitative PCR.
- VernacularTitle:荧光定量PCR分析FGFR2基因多态性与乳腺癌的相关性研究
- Author:
Li REN
1
,
2
;
Bin ZHANG
;
Xu-chen CAO
;
Ying CHEN
;
Jie GE
Author Information
- Publication Type:Journal Article
- MeSH: Breast Neoplasms; genetics; Female; Genetic Predisposition to Disease; Humans; Middle Aged; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; genetics; Receptor, Fibroblast Growth Factor, Type 2; genetics; Receptors, Estrogen; genetics
- From: Chinese Journal of Medical Genetics 2010;27(4):445-448
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association of fibroblast growth factor receptor 2 gene (FGFR2) rs2981582 polymorphism with breast cancer in Chinese women.
METHODSA case-control study was performed in 936 breast cancer patients and 471 patients with benign breast diseases by using a novel fluorescent quantitative PCR method.
RESULTSThe numbers and frequencies of genotypes CC, CT, and TT in the control group were 234(49.68%), 181(38.43%) and 56(11.89%) respectively. The numbers and frequencies of genotypes CC, CT, and TT in the breast cancer group were 426(44.56%), 400(41.84%) and 130(13.60%) respectively. And no significant difference was found between the two groups (P=0.183). However, stratified analysis found that the numbers and frequencies of genotypes CC, CT, TT in the estrogen receptor(ER) positive subgroup of breast cancer patients were 189(41.27%), 202(46.12%) and 67(14.63%) respectively, and significant difference was observed compared with control group (P=0.035).
CONCLUSIONAssociation was found in the single nucleotide polymorphism(SNP) of the rs2981582 locus of intron 2 in FGFR2 gene between the ER positive breast cancer patients and control patients with benign breast diseases. The fluorescent quantitative PCR is a specific, easy-to-operate, low-expense method and is suitable for SNP detection in large scale samples.
