Identification of the hemolytic transfusion reaction caused by lewis antibody using serological and molecular biological methods.
- Author:
Xiao-Zhen HONG
1
;
Xian-Guo XU
;
Fa-Ming ZHU
;
Kai-Rong MA
;
Li-Xing YAN
Author Information
1. Institute of Transfusion Medicine, Ministry of Health, Hangzhou, 310006, Zhejiang Province, China.
- Publication Type:Case Reports
- MeSH:
Adult;
Antibodies;
adverse effects;
immunology;
Blood Grouping and Crossmatching;
Female;
Fucosyltransferases;
genetics;
Genotype;
Hematologic Diseases;
Humans;
Lewis Blood-Group System;
immunology;
Serology;
Transfusion Reaction
- From:
Journal of Experimental Hematology
2008;16(5):1192-1195
- CountryChina
- Language:Chinese
-
Abstract:
To analyse the reason for one case of hemolytic transfusion reaction, antibodies in a patient's serum were identified using panel cells and Le (a-b-) phenotype cells, patient phenotype was identified by using anti-Le(a) and anti-Le(b) blood grouping reagents and the entire coding region of FUT3 gene was amplified by PCR and sequenced directly. The results showed that both IgM anti-Le(a) and anti-Le(b) antibodies were detected in patient's serum. Red cells was typed as Le (a-b-) phenotype and the FUT3 genotype was homozygote for non-functional le(59, 508) alleles. In conclusion, anti-Le(b) antibody can result in hemolytic transfusion reaction, FUT3 gene is homozygous for le(59, 508) allele resulting in Le (a-b-) phenotype.
