Aggregation after rehydration of lyophilized platelets.
- Author:
Jing-Han LIU
1
;
Fa-Qiang LU
;
Yuan ZHUANG
;
Ji CHE
;
Lin-Feng CHEN
Author Information
1. Department of Blood Transfusion, Center for Clinical Transfusion Medicine, The General Hospital of Chinese PLA, Beijing, 100853, China. jinghan-liu@301hospital.com.cn
- Publication Type:Journal Article
- MeSH:
Blood Platelets;
cytology;
drug effects;
metabolism;
Blood Preservation;
methods;
Freeze Drying;
methods;
Humans;
Platelet Aggregation;
physiology
- From:
Journal of Experimental Hematology
2006;14(4):812-815
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the aggregation of rehydrated-lyophilized platelets. The aggregation rate of fresh and rehydrated-lyophilized platelets were measured by using thrombin, ristocetin, ADP and collagen as inductors and APACT2 aggregameter; the effects of intra- and extra-cellular trehalose on maximum aggregation rate of rehydrated-lyophilized platelets were detected by using ADP as an inductor. The results showed that the aggregation rate of fresh platelets was all about 100%, while aggregation rate of rehydrated lyophilized platelets was (70.17 +/- 7.36)%, (15.3 +/- 2.81)%, (68.67 +/- 6.86)%, (64.67 +/- 11.6)% respectively, when the concentration of thrombin, ristocetin, ADP and collagen was 1 U/ml, 1.6 mg/ml, 20 micromol/L and 2 microg/ml. The maximum aggregation rates of rehydrated-lyophilized platelets in intra- and extra-cellular trehalose, extracellular trehalose and blank control groups were (66.0 +/- 4.69)%, (25.3 +/- 2.42)% and (11.5 +/- 1.87)% (P < 0.01), meanwhile there was significant difference of rehydrated-lyophilized platelet aggregation rate between intra- and extra-cellular trehalose and extracellular trehalose groups (P < 0.01). It is concluded that the concentrations of thrombin (1 U/ml), ristocetin (1.6 mg/ml), ADP (20 micromol/L) and collagen (2 microg/ml) are optimal for platelets aggregation tests, the internal and extracellular trehalose significantly enhance the aggregation of rehydrated-lyophilized platelets.
