Effects of parthenolide on high glucose-induced cell proliferation, NF-κB activation and MCP-1 expression in rat mesangial cells.
- Author:
Zhaozhong XU
1
,
2
;
Qianqian JIA
;
Hongyu LI
;
Jiancheng WANG
;
Yan ZHAO
;
Sijia CHEN
;
Haibo LONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents, Non-Steroidal; isolation & purification; pharmacology; Asteraceae; chemistry; Cell Proliferation; drug effects; Cells, Cultured; Chemokine CCL2; metabolism; Dose-Response Relationship, Drug; Glucose; administration & dosage; pharmacology; I-kappa B Proteins; metabolism; Mesangial Cells; cytology; metabolism; NF-KappaB Inhibitor alpha; NF-kappa B; antagonists & inhibitors; metabolism; Plants, Medicinal; chemistry; Rats; Sesquiterpenes; isolation & purification; pharmacology
- From: Journal of Southern Medical University 2013;33(10):1471-1473
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of parthenolide (PTL) on high glucose-stimulated cell proliferation, NF-κB activation and monocyte chemoattractant protein-1 (MCP-1) expression in rat mesangial cells (MCs).
METHODSMCs were cultured in normal glucose (5.6 mmol/L), high glucose (30 mmol/L), or high glucose with PTL. MTT assay was used to detect the cell proliferation. MCP-1 content in the supernatant was measured by ELISA, and the level of IκBα was detected by Western blotting to reflect NF-κB activity. EMSA method was used to measure the activation of NF-κB.
RESULTSMC proliferation, MCP-1 expression and NF-κB activation were significantly enhanced and the expression of NF-κB-binding protein IκBα was obviously reduced in cells cultured in high glucose. Application of PTL obviously abolished the effects of high glucose.
CONCLUSIONPTL can suppress high glucose-stimulated NF-κB activation and MCP-1 expression in rat MC. These data provide a theoretical basis for the clinical application of PTL in prevention and control of diabetic nephropathy.
