Bioinformatics analysis and identification of transcriptional regulation of human UCA1 gene.
- Author:
Mei XUE
1
,
2
;
Xu LI
;
Wei CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Binding Sites; genetics; Cell Line, Tumor; Computational Biology; CpG Islands; genetics; Cyclic AMP Response Element-Binding Protein; genetics; DNA Methylation; Humans; Promoter Regions, Genetic; genetics; Proto-Oncogene Proteins c-myb; genetics; metabolism; RNA, Long Noncoding; genetics; Software; Urinary Bladder Neoplasms; genetics; pathology
- From: Journal of Southern Medical University 2013;33(11):1596-1599
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the transcription factor binding sites and methylation in the promoter region of UCA1 gene.
METHODSUCA1 gene promoter was analyzed using CpG software to predict the CpG island. MatrixCatch and TFSEARCH were used to predict the potential transcription factor binding sites in UCA1 gene core promoter. ChIP assay was used to identify the transcription factors binding to UCA1 gene core promoter.
RESULTSUCA1 gene promoter contained no CpG island and was therefore a typical tissue-specific gene. There were 4 transcription factors associated with human cancers in UCA1 gene core promoter, but only one of them interacted with UCA1 gene core promoter.
CONCLUSIONThere is no CpG island in UCA1 gene promoter region, and the transcription factor c-Myb can specifically bind to the core promoter.
