The inhibitory effect of matrine on the growth of human colorectal cancer HT29 cells: an experimental observation.

Cheng CHANG; De-Li RAO; Xiao-Ming QIU; Hong WANG; Li XIONG

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The inhibitory effect of matrine on the growth of human colorectal cancer HT29 cells: an experimental observation.

Author: Cheng CHANG ¹ ; De-Li RAO ¹ ; Xiao-Ming QIU ¹ ; Hong WANG ¹ ; Li XIONG ²
Author Information

1. Department of MRI Room and Gastroenterology, Huangshi Central Hospital, Hubei College of Technology, Hubei 435000, China.
2. Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Hubei College of Technology, Hubei 435003, China.
Publication Type:Journal Article
MeSH: Alkaloids; pharmacology; Apoptosis; drug effects; Cell Proliferation; drug effects; HT29 Cells; Humans; Quinolizines; pharmacology
From: Chinese Journal of Integrated Traditional and Western Medicine 2014;34(1):62-65
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the inhibitory and inductive effect of matrine (MA) on human colorectal cancer HT29 cells.
METHODSMTT assay was used to determine the cell growth inhibitory rate in vitro . Changes of cell cycle and the apoptosis of HT29 cells before and after MA treatment were observed using flow cytometry and electron microscope.
RESULTSMTT showed that 2-32 mg/mL MA inhibited the proliferation of HT29 cells (P < 0.05) in a dose- and time-dependent manner. Cells treated by 4, 8, and 16 mg/mL MA at G0/G1 phase were obviously higher than those in the negative control group (P < 0.05), indicating that the cell cycle was arrested at G0/G1 phase. Morphological apoptosis of HT29 cells could be seen under transmission electron microscope.
CONCLUSIONMA inhibited the proliferation of HT29 cells, and its mechanism might be associated with stagnation at G0/G1 phase and inducing apoptosis of HT29 cells.