The proliferation of H1N1 subtype influenza viruses in A549 and BEAS-2B cells
10.3760/cma.j.issn.1003-9279.2011.03.015
- VernacularTitle:H1N1亚型流感病毒在A549和BEAS-2B细胞中复制情况的初步研究
- Author:
Shu-Mei ZOU
1
;
Jian-Fang ZHOU
;
Zi LI
;
Wen-Fei ZHU
;
Ye ZHANG
;
Le-Ying WEN
;
Yun ZHU
;
Xiao-Dan LI
;
Wei WANG
;
Yue-Long SHU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Influenza A virus;
Cells/ A549 and DEAS-2B;
Host
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(3):205-207
- CountryChina
- Language:Chinese
-
Abstract:
Objective Analyze the proliferation of different host H1N1 subtype influenza viruses in A549 and BEAS-2B cells. Methods Human, avain and swine three hosts of the H1N1 influenza viruses infected AS49 and BEAS-2B cells and analyze the characteristics of different periods after inocubation. Determine the receptor binding specificity of influenza virus by hemagglutination ( HA) test with RBCs with two types of receptor. And the receptors on surfaces of AS49 and BEAS-2B cells were tested by flow cytometry. Results The Cell Pathologic Effect(CPE) is obvious after 24 h inoculation in A549 cells by all the H1N1 influenza viruses, moreover,the peak hemagglutinin (HA) and 50% tissue culture cell infected dose (TCID50 ) titers was observed after 36 h of culturing in A549 cells. Otherwise, the CPE is not typical from 24 h-120 h inoculated by the same viruses and the HA,TCID50 titers were keep low all the periods in the BEAS-2B cell after inoculation. The receptor-binding preference of H1N1 viruses used in the study was screened by HA assay and some were found with 2-6-receptor binding affinity. Both SA a-2, 3Gal and SA a-2, 6Cal receptors were detected on A549 and BEAS-2B, furthermore, receptor density on AS49 cells was significantly higher than that of BEAS-2B cells. Conclusion A549 cells were susceptible to human, avian and swine H1N1 influenza viruses infection and permissively for viral replication. However, BEASE-2B cells with similar receptor pattern and epithelium-derived propriety as A549 cells were unsusceptible to their infection and replication. Possible host factors involved in effective viral infection and replication were needed further study.
