Effects of BIO-1211 on eosinophil chemotaxis, recruitment and mediator release.
- Author:
Xiao-yan ZHAO
1
;
Ji-qiang CHEN
;
Qiang-min XIE
;
Hui-fang TANG
;
Ru-lain BIAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Movement; drug effects; Chemotaxis, Leukocyte; drug effects; Dose-Response Relationship, Drug; Eosinophil Peroxidase; Eosinophils; drug effects; physiology; Integrin alpha4beta1; antagonists & inhibitors; physiology; Male; Oligopeptides; pharmacology; Peroxidases; secretion; Platelet Activating Factor; pharmacology; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2003;32(4):279-291
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of very late antigen(VLA) antagonist BIO-1211 on eosinophil chemotaxis, recruitment and mediator release.
METHODSEosinophil chemotaxis was induced by platelet activating factor(PAF) in vitro and eosinophil recruitment and release were determined in vivo.
RESULTVLA antagonist BIO-1211 inhibited eosinophil chemotaxis induced by PAF. The inhibitory rates at 4x10(-11), 4x10(-10), 4x10(-9) mol x L(-1) were 24.9%, 29.9%, and 31.3%, respectively. Pretreatment by BIO-1211 1, 3 and 10 mg x kg(-1) intraperitoneally inhibited the recruitment of eosinophils in PAF in the rat induced by Sephadex in a dose dependent manner. Inhibitory rates were 60.3%, 68.9%, and 72.9%(P<0.05), respectively. BIO-1211 did not inhibit eosinophil peroxidase(EPO) release from eosinophils.
CONCLUSIONBIO-1211 inhibits eosinophil chemotaxis and recruitment, alleviates local inflammation, and may represent a new type of drug for allergic diseases.
