Effect of eucalyptus globulus oil on activation of nuclear factor-kappaB in THP-1 cells.
- Author:
Jian-ya ZHOU
1
;
Fa-di TANG
;
Guo-gen MAO
;
Jie SHAO
;
Yan WANG
;
Ru-lian BIAN
Author Information
- Publication Type:Journal Article
- MeSH: Active Transport, Cell Nucleus; drug effects; Blotting, Western; Cell Line; Dose-Response Relationship, Drug; Eucalyptus; chemistry; Humans; Lipopolysaccharides; pharmacology; NF-kappa B; antagonists & inhibitors; metabolism; Plant Oils; pharmacology; Tosyllysine Chloromethyl Ketone; pharmacology
- From: Journal of Zhejiang University. Medical sciences 2003;32(4):315-326
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of eucalyptus globulus oil on the activity of nuclear factor-kappaB(NF-kappaB) in THP-1 cell line.
METHODSTHP-1 cells were cultured with or without eucalyptus globulus oil at different concentrations (1, 10, 100 mg x L(-1), 30 min) before being stimulated with lipopolysaccharide (LPS, 1 mg x L(-1), 30 min). The location of NF-kappaB p65 subunit (NF-kappaB/p65) in THP-1 cells was detected by indirect immunofluorescence and laser scanning confocal microscope. The expression of NF-kappaB/p65 in nuclei was measured by Western-blot analysis.
RESULTThe FITC-label NF-kappaB/p65 was mainly located in the nuclei after THP-1 cells were stimulated with LPS. Whereas, no fluorescence were seen in the nuclei of cells pretreated with eucalyptus globulus oil. This effect on NF-kappaB/p65 nuclear translocation was in a concentration dependent manner.
CONCLUSIONEucalyptus globulus oil inhibits the nuclear translocation of NF-kappaB induced by LPS in THP-1 cells.
