Cloning and expression analysis of kaurenoic acid oxidase gene in Tripterygium wilfordii.
10.19540/j.cnki.cjcmm.20161222.034
- Author:
Yi-Feng ZHANG
1
;
Ping SU
2
;
Tian-Yuan HU
1
;
Jia-Wei ZHOU
1
;
Hong-Yu GUAN
1
;
Wei GAO
1
;
Lu-Qi HUANG
3
Author Information
1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
2. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
3. State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica,Chinese Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
KAO;
Tripterygium wilfordii;
bioinformatics analysis;
cloning;
expression analysis
- From:
China Journal of Chinese Materia Medica
2017;42(1):88-93
- CountryChina
- Language:Chinese
-
Abstract:
Kaurenoic acid oxidase involved in biosynthesis pathway of gibberellin. According to the transcriptome database, the specific primers were designed and used in cloning the full-length cDNA of TwKAO, the bioinformatic analysis of the sequence was performed. The qRT-PCR were used to detect the expression level of TwKAO after MeJA treatment.The full-length cDNA of the TwKAO was 1 874 bp encoding a polypeptide of 487 amino acids.The calculate molecular weight was about 56.02 kDa,and the theoretical isoelectric point (pI) was 8.89. The relative expression level of TwKAO was deduced by MeJA and reached the highest at 12 h after the treatment.Plant tissue expression analysis indicated that, TwKAO expressed the highest in leaves,while lowest in roots.For the first time, we cloned and analyzed the expression characteristics of TwKAO, which laid a foundation for deep analysis of growing development and terpenoid secondary metabolites in T. wilfordii.
