Inhibitory effects of alpha-zearalenol on angiotensin II-induced integrin beta3 mRNA via suppression of nuclear factor-kappaB.
- Author:
Su-Min LI
1
;
Xiao-Ming WANG
;
Jin QIU
;
Qin SI
;
Heng-Yi GUO
;
Ren-Yu SUN
;
Qi-Xia WU
Author Information
- Publication Type:Journal Article
- MeSH: Angiotensin II; antagonists & inhibitors; Cells, Cultured; Endothelial Cells; drug effects; metabolism; Endothelium, Vascular; drug effects; metabolism; Estradiol; pharmacology; Female; Gene Expression Regulation; Humans; Integrin beta3; biosynthesis; genetics; NF-kappa B; antagonists & inhibitors; physiology; Nitric Oxide; antagonists & inhibitors; Phytoestrogens; pharmacology; RNA, Messenger; metabolism; Receptors, Estrogen; antagonists & inhibitors; Zeranol; analogs & derivatives; pharmacology
- From: Biomedical and Environmental Sciences 2005;18(5):314-320
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effect of alpha-zearalenol on angiotensin II-induced beta3 integrin mRNA expression in human umbilical vein endothelial cells (HUVECs).
METHODSThe mRNA level in integrin beta3 was determined by reverse transcription-polymerase chain reaction. Endothelial NF-kappaB activity was determined by the luciferase activity assay of plasmid NF-kappaB-LUC.
RESULTSThe angiotensin II-induced beta3 integrin mRNA expression was inhibited by alpha-zearalenol and 17beta-estradiol (10 nmol/L -1 micromol/L), but not influenced by ICI 182, 780, a pure competitive antagonist for estrogen receptor or a nitric oxide inhibitor Nomega-Nitro-L-arginine methyl ester hydrochloride. Alpha-zearalenol and 17beta-estradiol suppressed the angiotensin II-induced activation of NF-kappaB in endothelial cells.
CONCLUSIONAlpha-zearalenol inhibits angiotensin II-induced integrin beta3 mRNA expression by suppressing NF-kappaB activation in endothelial cells.
