Der f 2 activates phospholipase D in human T lymphocytes from Dermatophagoides farinae specific allergic individuals: Involvement of protein kinase C-alpha.
- Author:
Jae Won OH
1
;
Eun Young KIM
;
Bon Suk KOO
;
Ha Baik LEE
;
Ki Sung LEE
;
Yong Seok KIM
;
Joong Soo HAN
Author Information
1. Department of Pediatrics, College of Medicine, Hanyang University Seoul 133-791, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
PKC-alpha, allergy;
Der f2;
phospholipase D;
T lymphocytes
- MeSH:
Adolescent;
Adult;
Animals;
Antigens, Dermatophagoides/*immunology;
Dermatophagoides farinae/*immunology;
Female;
Humans;
Hypersensitivity, Immediate/*enzymology/*immunology;
Male;
Phospholipase D/*metabolism;
Protein Kinase C/antagonists & inhibitors/*physiology;
Research Support, Non-U.S. Gov't;
Skin Tests;
T-Lymphocytes/*enzymology/immunology;
Tetradecanoylphorbol Acetate/*analogs & derivatives/pharmacology;
Up-Regulation
- From:Experimental & Molecular Medicine
2004;36(5):486-492
- CountryRepublic of Korea
- Language:English
-
Abstract:
The major house-dust mite allergen, Der f 2, stimulates the phospholipase D (PLD) in T lymphocytes from Dermatophagoides farinae specific allergic individuals. PLD activity increased more than two-fold in T cells from allergic patients compared with those cells from normal controls with maximal responses within 30 min after exposure of Der f 2. A well-known PLD activator PKC-alpha was found to be translocated to membrane from cytosol in Der f 2-treated T cells from Dermatophagoides farinae specific allergic individuals. Down-regulation of PKC-alpha with phorbol myristate acetate pretreatment for 24 h abolished Der f 2-induced PLD activation. Ro 320432, PKC inhibitor also reduced the effects of Der f 2-induced PLD activation suggesting that PKC-alpha acts as upstream activator of PLD in Der f 2-treated T cells. Taken together, the present data suggest that Der f 2 can stimulate PLD activity through the PKC-alpha activation in T cells from Dermatophagoides farinae allergic individuals
