Autophagy Activity in Pulmonary Metastatic Tumor Tissues from Colorectal Cancer: A Pilot Study.
10.3349/ymj.2014.55.6.1484
- Author:
Young Sam KIM
1
;
Ju Hye SHIN
;
Mi Kyung BAE
;
Chang Young LEE
;
Dae Joon KIM
;
Kyung Young CHUNG
;
Jin Gu LEE
Author Information
1. Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Colorectal cancer;
metastasis;
treatment failure;
autophagy
- MeSH:
Adaptor Proteins, Signal Transducing;
Aged;
*Autophagy;
Blotting, Western;
Colorectal Neoplasms/*metabolism/*pathology;
Female;
Humans;
Lung Neoplasms/*metabolism/*pathology;
Male;
Microfilament Proteins/*metabolism;
Microscopy, Electron, Transmission;
Microtubule-Associated Proteins;
Middle Aged;
Neoplasm Recurrence, Local;
Pilot Projects;
Proteins;
Retrospective Studies;
Treatment Failure;
Tumor Markers, Biological/metabolism
- From:Yonsei Medical Journal
2014;55(6):1484-1488
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Autophagy has been reported to be involved in treatment failure in tumor. We aimed to evaluate autophagy activity in tumor tissue and compare them between the recurrence and non-recurrence groups. MATERIALS AND METHODS: We analyzed expressions of autophagy-related proteins in tumor tissues which were obtained from pulmonary metastases of colorectal cancer patients by Western blot. We also analyzed autophagosomes by transmission electron microscopy. RESULTS: Tumor tissues from recurrence group showed increased levels of LC3B-II, decreased levels of p62/SQSTM1, and also a marked accumulation of autophagosomes compared with tissues from non-recurrence group. CONCLUSION: The present study suggests that autophagy may be associated with treatment failure of metastatic colorectal cancer.
