Involvement of the CXC chemokines Mig and IP-10 in response to M. bovis BCG in mice.
- Author:
Seong Tshool HONG
1
;
Jung Gil CHO
;
Hwang Ho LEE
Author Information
1. Department of Microbiology, Chonbuk National University Medical School, Chonju, 561-182, South Korea. hwangho@moak.chonbuk.ac.kr
- Publication Type:Original Article
- MeSH:
Animals;
Chemokines;
Chemokines, CXC*;
Cooperative Behavior;
Hypersensitivity;
Interleukin-12;
Kinetics;
Liver;
Lung;
Mice*;
Monocytes;
Mycobacterium bovis*;
RNA, Messenger;
Spleen;
T-Lymphocytes
- From:Journal of the Korean Society for Microbiology
2000;35(2):117-127
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The non-ELR-containing CXC chemokines Mig and IP-10 have been shown to function as chemotactic cytokines for activated T lymphocytes. In this study, we examined the potential involvement of Mig and IP-10 in antimycobacterial response of mice immunized or infected with M. bovis BCG. The accumulation of Mig and IP-10 mRNA in resident peritoneal monocytes (RPMPHI) was slightly reduced by stimulation with vBCG, and the degree was greater for 24 hr culture even though IFN-gamma was added. Expression of Mig, IP-10, and IFN-gamma in 24 hr delayed-type hypersensitivity (DTH) response was stronger in vBCG-immune mice than in the non-immune. The increase of DTH measured by foot-pad thickness appears to be clearly related to the levels of chemokines Mig and IP10 messages and those of IFN-gamma and IL-12. Stimulation with vBCG for 2 days decreased or completely dropped the levels of Mig message in non-immune or immune splenocytes, respectively, whereas IP-10 message was slightly decreased in 2 days culture. Moreover, messages for IL-12 (p40) showed similar kinetics for Mig. The levels of Mig and IP-10 mRNA during the course of infection with BCG were not readily changed in lungs, livers, and spleens from BCG-infected mice. Although there was no obvious changes of Mig and IP-10 messages in the target organs during infection process, we found that the infection progressed over the first 3 wk before being contained by the emerging immune response suggested from detectable amount of IFN-gamma mRNA around this time. In view of selectivity of chemokines Mig and IP-10 for activated T cells, these data suggest that chemokine Mig and IP-10, especially in collaboration with IL-12 and IFN-gamma, may play a role as T cell recruiters in immune response against mycobacterial infection.
