Differential antitumor effects of sequence-dependent model in tumor cell line: association with peroxiredoxin.
- Author:
Hee Young CHO
1
;
Gyeongwon LEE
;
Junghun KANG
;
Young Sool HAH
;
Joung Soon JANG
Author Information
1. Department of Internal Medicine, GyeongSang National University Hospital, Jinju, Korea.
- Publication Type:Original Article
- Keywords:
Cervix cancer;
Sequence-dependent;
Taxol;
Doxorubicin;
Peroxiredoxin
- MeSH:
Appointments and Schedules;
Blotting, Western;
Cell Cycle;
Cell Line;
Cell Line, Tumor*;
Cyclin D1;
Doxorubicin;
HeLa Cells;
Humans;
Paclitaxel;
Parents;
Peroxiredoxins*;
Uterine Cervical Neoplasms
- From:Korean Journal of Gynecologic Oncology
2007;18(4):326-332
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The efficacy of two-drug combination treatment may be schedule-dependent. We investigated a simulated in-vitro interaction between taxol and doxorubicin in a Cervical cancer cell line HeLa and the role of peroxiredoxin in cytotoxicity. METHODS: Two contradicting schedules of two drugs (taxol followed by doxorubicin or vice versa) were compared each other in terms of cytotoxicity in parental HeLa cell line and the peroxiredoxin (prx)-overexpressing variant. Cytotoxic activity was determined by MTT assay. Cell cycle pertubation was evaluated by flow cytometric analysis. Protein levels were determined by western blot. RESULTS: The sequential treatment of taxol followed by doxorubicin (T--
