Interleukin-17 Enhances Germinal Center Formation and Immunoglobulin G1 Production in Mice.
10.4078/jrd.2017.24.5.271
- Author:
Jennifer LEE
1
;
Seon Young LEE
;
Chang Min KANG
;
Joo Yeon JHUN
;
Ji Hun KIM
;
Mi La CHO
;
Sung Hwan PARK
;
Ho Youn KIM
;
Seung Ki KWOK
Author Information
1. The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Korea. seungki73@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Interleukin-17;
B cell;
Germinal center
- MeSH:
Animals;
B-Lymphocytes;
Cell Differentiation;
Cytidine Deaminase;
Enzyme-Linked Immunosorbent Assay;
Flow Cytometry;
Germinal Center*;
Immunity, Humoral;
Immunoglobulin G;
Immunoglobulins*;
In Vitro Techniques;
Interleukin-17*;
Interleukin-4;
Interleukins;
Mice*;
Plasma Cells;
Recombination, Genetic
- From:Journal of Rheumatic Diseases
2017;24(5):271-278
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Interleukin (IL)-17 is a pro-inflammatory cytokine that has pleiotropic effects on multiple target cells and thereby contributes to the development of immune-mediated inflammatory disorders. However, the role of IL-17 in the humoral immune response has not been clearly elucidated. METHODS: Mice deficient in IL-17A (IL-17A knockout [KO] mice) and wild type (WT) C57BL/6 mice were compared. Distinct B cell (mature/precursor and marginal zone/follicular) and plasma cell populations were compared using fluorescence-activated cell sorting (FACS) and confocal immunostaining. Immunoglobulin production was assessed by enzyme-linked immunosorbent assay. RESULTS: There was no difference in B cell and plasma cell populations between IL-17A KO and WT mice. However, after T cell-dependent antigen challenge, IL-17A KO mice produced lower levels of immunoglobulin (Ig)G1 than wild-type animals. IL-17A KO mice also showed reduced germinal center (GC) formation and lower expression of activation-induced cytidine deaminase, the essential enzyme for class switch recombination (CSR). IL-17 had no effect on the proliferation or survival of naïve B cells in in vitro functional studies. However, IL-17 treatment promoted naïve B cell differentiation into plasma cells in synergy with IL-4, although IL-17 alone had no effect. CONCLUSION: Our findings suggest that IL-17 contributes to the humoral immune response by enhancing GC formation, CSR to IgG1, and plasma cell differentiation in synergy with IL-4.
