The Effects of Antioxidants and Nitric Oxide Modulators on Hepatic Ischemic-Reperfusion Injury in Rats.
10.3346/jkms.2002.17.4.502
- Author:
Joong Eui RHEE
1
;
Sung Eun JUNG
;
Sang Do SHIN
;
Gil Joon SUH
;
Dong Young NOH
;
Yeo Kyu YOUN
;
Seung Keun OH
;
Kuk Jin CHOE
Author Information
1. Department of Emergency Medicine, Seoul National University College of Medicine, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea. sejung@plaza.snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Reperfusion Injury;
Nitric Oxide;
Antioxidants;
Tumor Necrosis Factor;
Ascorbic Acid
- MeSH:
Animals;
Antioxidants/*metabolism;
Arginine/metabolism;
Ascorbic Acid/metabolism;
Catalase/metabolism;
Enzyme Inhibitors/metabolism;
Liver/*metabolism/pathology;
Male;
Malondialdehyde/metabolism;
Nitric Oxide/*metabolism;
Nitric Oxide Synthase/metabolism;
Nitric Oxide Synthase Type II;
Nitrites/blood;
Nitroarginine/metabolism;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury/*metabolism;
Tumor Necrosis Factor-alpha/metabolism;
Vitamin E/metabolism
- From:Journal of Korean Medical Science
2002;17(4):502-506
- CountryRepublic of Korea
- Language:English
-
Abstract:
Ischemic-reperfusion injury (IRI) is thought to be caused by oxygen radicals. Nitric oxide (NO) also has been thought to play a key role in IRI. This experiment was designed to evaluate the effects of antioxidants and NO supplement on hepatic IRI. Male Sprague-Dawley rats were divided into five groups: a sham operation group, a group with IRI, and three groups with vitamin C combined with vitamin E (VC&VE), L-arginine and N(G)-nitro-L-arginine (NNLA) injected after IRI. IRI was induced by clamping of the porta hepatis for 30 minutes and then by declamping. To prevent mesenteric blood congestion, a porto-systemic shunt had been made four weeks before the portal clamping. Biochemical assays of TNF-alpha level and NO2- level in the blood, malondialdehyde level, catalase activity and NO synthase activity in the liver tissue were performed. The results were as follows: IRI increased the malondialdehyde level and exhausted the catalase activity remarkably. VC&VE lowered the malondialdehyde levels and protected against catalase exhaustion, but had no significant effect on the NO production. L-arginine had a definite antioxidant effect, which was much weaker than that of VC&VE. In conclusion, antioxidants and a supplement of NO protected the liver tissue against IRI.
