Inhibition of Neointima Formation by Anti-Vascular Endothelial Growth Factor and Receptor-1 Peptides in a Balloon-Injured Rat Carotid Artery.
10.4070/kcj.2007.37.10.475
- Author:
Jong Min LEE
1
;
Keon Woong MOON
;
Ki Dong YOO
;
Sung Ho HER
;
Hee Jeoung YOON
;
Seung Won JIN
;
Doo Soo JEON
;
Ho Joong YOUN
;
Wook Sung CHUNG
;
Ki Bae SEUNG
;
Chul Min KIM
;
Jae Hyung KIM
;
Kyu Bo CHOI
;
Soon Jo HONG
Author Information
1. Division of Cardiology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea. expert98@dreamwiz.com
- Publication Type:Original Article
- Keywords:
Vascular endothelial growth factors;
Vascular endothelial growth factor receptor-1;
Peptides;
Carotid artery injuries
- MeSH:
Animals;
Carotid Arteries*;
Carotid Artery Injuries;
Chemokine CCL2;
Endothelial Growth Factors*;
Gyeongsangbuk-do;
Humans;
Hyperplasia;
Inflammation;
Interleukin-6;
Korea;
Male;
Neointima*;
Peptides*;
Rats*;
Rats, Sprague-Dawley;
Up-Regulation;
Vascular Endothelial Growth Factor A;
Vascular Endothelial Growth Factor Receptor-1;
Vascular Endothelial Growth Factors
- From:Korean Circulation Journal
2007;37(10):475-482
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND AND OBJECTIVES: Vascular endothelial growth factor (VEGF) is a potent endothelial cell-specific mitogen. This study was undertaken to test the hypothesis that the neointima hyperplasia induced by a balloon injury is inhibited by blocking VEGF and VEGF receptor-1 (VEGFR-1) with anti-VEGF peptides. Materials and Methods: Anti-VEGF RRKRRR peptide (dRK6) and anti-VEGFR-1 peptide (anti-flt-1) were synthesized at Pohang University of Science and Technology, Korea. Male Sprague-Dawley rats, weighing 300-350 g, were subcutaneously injected 0.5 mg/kg of dRK6 or 0.5 mg/kg of anti-flt-1, dissolved in phosphate buffer solution, 2 days before induction of a carotid balloon-injury, and then daily in the same manner post carotid balloon injury for 2 weeks. RESULTS: Neointima formation was suppressed in both the dRK6 and anti-flt-1 groups compared to that in the untreated controls at 2 weeks post carotid balloon-injury (neointimal area; control group 0.44+/-0.09 mm2, dRK6 group 0.25+/-0.05 mm2, anti-flt-1 group 0.19+/-0.05 mm2, p<0.01). Anti-flt-1 peptide and dRK6 reduced the numbers of proliferative bromodeoxyuridine-labeled cells in the neointima (control group 16.4+/-10.6%, dRK6 group 3.7+/-2.1%, anti-flt-1 group 5.9+/-3.4%, p<0.05). In addition, an inflammatory response, as determined by monocyte chemoattractant protein-1 and interleukin-6 upregulation, which was evident in the controls, was inhibited by both dRK6 and anti-flt-1. CONCLUSION: This study suggests anti-vascular endothelial growth factor peptides can reduce the inflammation and neointima formation in balloon injured rat carotid arteries.
