Identification and antigenic site analysis of foot-and-mouth disease virus from pigs and cattle in Korea.
- Author:
Jae Ku OEM
1
;
Kwang Nyeong LEE
;
In Soo CHO
;
Soo Jeong KYE
;
Jee Yong PARK
;
Jong Hyeon PARK
;
Yong Joo KIM
;
Yi Seok JOO
;
Hee Jong SONG
Author Information
1. National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang 430-824, Korea. jku0622@nvrqs.go.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
antigenic site;
foot-and-mouth disease virus;
FMD;
O/SKR/2002
- MeSH:
Amino Acid Sequence;
Animals;
Antibodies, Viral/blood;
Base Sequence;
Capsid Proteins/genetics/*immunology;
Cattle;
Cattle Diseases/epidemiology/*virology;
Cluster Analysis;
Disease Outbreaks/*veterinary;
Enzyme-Linked Immunosorbent Assay/veterinary;
Epitopes/analysis;
Foot-and-Mouth Disease/epidemiology/*virology;
Foot-and-Mouth Disease Virus/genetics/*immunology;
Korea/epidemiology;
Molecular Sequence Data;
Phylogeny;
RNA, Viral/chemistry/genetics;
Reverse Transcriptase Polymerase Chain Reaction/veterinary;
Sequence Alignment;
Swine;
Swine Diseases/epidemiology/*virology
- From:Journal of Veterinary Science
2005;6(2):117-124
- CountryRepublic of Korea
- Language:English
-
Abstract:
From May to June 2002, a total of 16 foot-and mouth disease (FMD) outbreaks due to the serotype O virus, Pan Asia strain, were recorded in Korea. The viruses were identified by antigen ELISA, RT-PCR and sequence analysis. The overall nucleotide sequence divergence of the VP1 region among the 4 isolates in 2002 was 0 to 1.4%, but between O/SKR/2002 and O/SKR/2000 isolates was 1.9-4.9%. Phylogenetic analysis with the some known strains from East Asian countries showed that the 4 Korean isolates in 2002 formed one distinct cluster, which different from clusters of Korean isolates in 2000, with in the same lineage of the ME-SA topotype strains. Deduced amino acid sequences around neutralizable antigenic site on VP1 site of O/SKR/2002 isolates were aligned and compared with other strains. At the antigenic site 1, the replacements of the critical amino acid residues at position 144 from V to L and at position 152 from A to T were observed in O/SKR/2002 viruses. For antigenic site 2 and 4, there were not significant variations in general. At the antigenic site 3, the substitutions of amino acid residues were present at positions 54 and 56 in O/SKR/2002 isolates and an alternative residue I at position 54 are observed only at the sequence of O/SKR/AS/2002 (cow) virus. And the substitution (L-->P) of significant residue at position 144 was detected at the amino acid sequence of the O/SKR/2002 (cow) virus.
