Proteomic analyses of cervical cancer tissues by two-dimensional gel electrophoresis and mass spectrometry.
- Author:
Sung Ha LEE
1
;
Su Mi BAE
;
Ok Kyoung KIM
;
Hyun Jung KIM
;
Eun Kyung PARK
;
Hae Nam LEE
;
Yong Wook KIM
;
Duck Yeong RO
;
Joon Mo LEE
;
Sung Eun NAMKOONG
;
Young Lae CHO
;
Gye Hyun NAM
;
Byung Don HAN
;
Yong Wan KIM
;
Chong Kook KIM
;
Woong Shick AHN
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea. ahnws@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Cervical cancer;
Squamous cell carcinoma;
Two-dimensional gel electrophoresis (2-DE);
Matrix assisted laser desorption/ ionization-time of flighting (MALDI-TOF)
- MeSH:
14-3-3 Proteins;
Actins;
Aflatoxin B1;
Aldehyde Reductase;
Annexin A2;
Carcinoma, Squamous Cell;
Cervix Uteri;
Databases, Protein;
Down-Regulation;
Electrophoresis, Gel, Two-Dimensional*;
Electrophoresis, Polyacrylamide Gel;
Female;
Hot Temperature;
Humans;
Isoelectric Focusing;
Keratin-13;
Keratin-19;
Keratin-20;
Mass Spectrometry*;
Muscle Proteins;
Myosin Light Chains;
Phospholipid Transfer Proteins;
Phosphopyruvate Hydratase;
Receptors, Tumor Necrosis Factor;
Running;
Serine;
Shock;
Sodium Dodecyl Sulfate;
Tropomyosin;
Up-Regulation;
Uterine Cervical Neoplasms*
- From:Korean Journal of Obstetrics and Gynecology
2005;48(7):1686-1697
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Comparison of protein expressions by two-dimensional gel electrophoresis (2-DE) in normal cervix and squamous cell carcinoma tissues in Korean women. METHODS: Normal cervix and squamous cell carcinoma tissues were solubilized with 2-DE buffer and the first dimension of PROTEAN IEF CELL, isoelectric focusing (IEF), was performed using pH3-10 linear IPG strips of 17 cm. And then running 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and sliver stain. Scanned image was analyzed using PDQuest 2-D softwareTM. Protein spot spectrum was identified by assisted laser desorption/ionization-time of fighting (MALDI-TOF) and the protein mass spectrum identifications were performed by searching protein databases of Swiss-prot/TrEMBL, Mascot and MS-FIT. RESULTS: We found 9 up-regulation proteins (Alpha enolase, Keratin 19 type I, Keratin 20 type I, Keratin 13 type I, beta-actin, Aflatoxin B1 aldehyde reductase 1, Annexin A2, Squamous cell carcinoma antigen 2, unknown), 7 down-reguation proteins (Annexin 1, Myosin regulatory light chain 2, 14-3-3 protein epsilon, Heat shock 27 kDa protein, Hypothetical protein (DKFZP434C1715), Tumor necrosis factor receptor superfamily member 13B, Smoth muscle protein 22-alpha) and 6 up and down-regulation proteins (Tropomyosin 1, Tropomyosin 2, Tropomyosin 3, Serine (or cysteine) proteinase inhibitor, Phosphatidylinositol transfer protein alpha isoform, Src homology 3 domain-containing protein HIP-55) between normal cervix and squamous cell carcinoma cell tissues. CONCLUSION: 2-DE offers total protein expressions between normal cervix and squamous cell carcinoma cell tissues, and searching of differently expressed protein for the diagnostic markers of squamous cell carcinoma tissue.
