Neuroprotective Effect of Resveratrol on Acute Brain Ischemia Reperfusion Injury by Measuring Annexin V, p53, Bcl-2 Levels in Rats.
10.3340/jkns.2015.58.6.508
- Author:
Ceren KIZMAZOGLU
1
;
Hasan Emre AYDIN
;
Ismail Ertan SEVIN
;
Orhan KALEMCI
;
Nurullah YUCEER
;
Metin Ant ATASOY
Author Information
1. Department of Neurosurgery, Katip Celebi University Izmir Ataturk Training and Research Hospital, Izmir, Turkey.
- Publication Type:Original Article
- Keywords:
Resveratrol;
Brain Ischemia;
Annexin V;
Bcl-2;
p53
- MeSH:
Animals;
Annexin A5*;
Apoptosis;
Brain Ischemia*;
Brain*;
Carotid Artery, Common;
Cell Count;
Cerebral Infarction;
Ischemia;
Neuroprotective Agents*;
Rats*;
Reperfusion Injury*;
Reperfusion*;
Skin
- From:Journal of Korean Neurosurgical Society
2015;58(6):508-512
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Cerebral ischemia is as a result of insufficient cerebral blood flow for cerebral metabolic functions. Resveratrol is a natural phytoalexin that can be extracted from grape's skin and had potent role in treating the cerebral ischemia. Apoptosis, a genetically programmed cellular event which occurs after ischemia and leads to biochemical and morphological changes in cells. There are some useful markers for apoptosis like Bcl-2, bax, and p53. The last reports, researchers verify the apoptosis with early markers like Annexin V. METHODS: We preferred in this experimental study a model of global cerebral infarction which was induced by bilateral common carotid artery occlusion method. Rats were randomly divided into 4 groups : sham, ischemia-reperfusion (I/R), I/R plus 20 mg/kg resveratrol and I/R plus 40 mg/kg resveratrol. Statistical analysis was performed using Sigmastat 3.5 ve IBM SPSS Statistics 20. We considered a result significant when p<0.001. RESULTS: After administration of resveratrol, Bcl-2 and Annexin levels were significantly increased (p<0.001). Depending on the dose of resveratrol, Bcl2 levels increased, p53 levels decreased but Annexin V did not effected. P53 levels were significantly increased in ishemia group, so apoptosis is higher compared to other groups. CONCLUSION: In the acute period, Annexin V levels misleading us because the apoptotic cell counts could not reach a certain level. Therefore we should support our results with bcl-2 and p53.
