The Effect of Pulsed Radiofrequency Applied to the Peripheral Nerve in Chronic Constriction Injury Rat Model.
10.5535/arm.2015.39.5.667
- Author:
Jun Beom LEE
1
;
Jeong Hyun BYUN
;
In Sung CHOI
;
Young KIM
;
Ji Shin LEE
Author Information
1. Department of Physical and Rehabilitation Medicine, Research Institute of Medical Sciences, Chonnam National University Medical School & Hospital, Gwangju, Korea. drchoiis@naver.com
- Publication Type:Original Article
- Keywords:
Neuralgia;
Pulsed radiofrequency treatment;
Peripheral nerves;
Tumor necrosis factor-alpha
- MeSH:
Animals;
Blotting, Western;
Constriction*;
Hot Temperature;
Humans;
Immunohistochemistry;
Male;
Models, Animal*;
Neuralgia;
Peripheral Nerve Injuries;
Peripheral Nerves*;
Pulsed Radiofrequency Treatment;
Rats*;
Rats, Sprague-Dawley;
Sciatic Nerve;
Spinal Cord;
Tumor Necrosis Factor-alpha
- From:Annals of Rehabilitation Medicine
2015;39(5):667-675
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To investigate the effect of pulsed radiofrequency (PRF) applied proximal to the injured peripheral nerve on the expression of tumor necrosis factor-alpha (TNF-alpha) in a neuropathic pain rat model. METHODS: Nineteen male Sprague-Dawley rats were used in the study. All rats underwent chronic constriction injury (CCI) procedure. After 7 days of CCI, withdrawal frequency of affected hind paw to mechanical stimuli and withdrawal latency of affected hind paw to heat stimulus were measured. They were randomly divided into two groups: group A, CCI group (n=9) and group B, CCI treated with PRF group (n=10). Rats of group B underwent PRF procedure on the sciatic nerve. Withdrawal frequency and withdrawal latency were measured at 12 hours, and 7 days after PRF. Immunohistochemistry and Western blot analysis were performed using a TNF-alpha antibody. RESULTS: Before PRF, withdrawal frequency and withdrawal latency were not different in both groups. After PRF, withdrawal frequency decreased and withdrawal latency prolonged over time in group B. There was significant interaction between time and group for each withdrawal frequency and withdrawal latency. Group B showed decreased TNF-alpha immunoreactivity of the spinal cord and sciatic nerve at 7 days. CONCLUSION: PRF applied proximal to the peripheral nerve injury is potentially helpful for the reduction of neuropathic pain by neuromodulation of inflammatory markers.
